目的 研究铁皮石斛多糖(DOP)对顺铂(cisplatin)致雄性小鼠生殖损伤的影响。方法 40只雄性昆明小鼠随机分为8组:对照组(control)、低剂量多糖组(LD-DOP)、中剂量多糖组(MD-DOP)、高剂量多糖组(HD-DOP)、顺铂组、顺铂-低剂量多糖组(cisplatin-LD-DOP)、顺铂-中剂量多糖组(cisplatin-MD-DOP)、顺铂-高剂量多糖组(cisplatin-HD-DOP),每组各5只小鼠。顺铂组与顺铂-低、中、高剂量多糖组连续5 d腹腔注射2.5 mg·kg-1·d-1顺铂;对照组与低、中、高剂量多糖组注射等体积生理盐水。末次注射24 h后,多糖组与顺铂-多糖组小鼠分别灌胃DOP 100 mg·kg-1·d-1(低剂量)、200 mg·kg-1·d-1(中剂量)、400 mg·kg-1·d-1(高剂量),对照组与顺铂组灌胃等体积生理盐水,灌胃35 d后处死小鼠,观察DOP对模型小鼠精子数量、精子功能、睾丸组织病理学变化和睾丸氧化应激水平的影响。结果 与顺铂组相比,模型小鼠灌胃不同剂量的DOP后,其睾丸和附睾系数提高(P<0.01)、精子数量增加、精子功能改善;睾丸组织SOD酶活力增强(P<0.01)、MDA含量下降(P<0.01),睾丸组织病理损伤得到明显恢复。与顺铂-低剂量多糖组小鼠相比,顺铂-中、高剂量多糖组小鼠的精子数量增加、精子功能改善、睾丸组织氧化损伤程度下降、睾丸组织病理学损伤减轻。结论 连续灌胃DOP可使顺铂模型小鼠精子数量增加,精子功能改善,睾丸组织氧化损伤程度下降、睾丸组织病理学损伤减轻,说明DOP对小鼠生殖系统有保护作用,且存在剂量效应。
Abstract
OBJECTIVE To study the effects of polysaccharides from Dendrobium candidum Kimura et Migo (DOP) on reproductive damage in male mice induced by cisplatin. METHODS Forty male Kunming mice were randomly divided into eight groups: control group, DOP low-dose group (LD-DOP), DOP medium-dose group (MD-DOP), DOP high-dose group (HD-DOP), cisplatin group, cisplatin-low-dose DOP group (cisplatin-LD-DOP), cisplatin-medium-dose DOP group (cisplatin-MD-DOP), and cisplatin-high-dose DOP group (cisplatin-HD-DOP), 5 mice in each group. The cisplatin group and cisplatin-LD/MD/HD-DOP group were intraperitoneally injected with 2.5 mg·kg-1·d-1 cisplatin for 5 d, control group and LD/MD/HD-DOP group were intraperitoneally injected with equal volume of saline. After 24 h of the last injection, the 3 DOP groups and cisplatin-DOP group were intragastrically administrated with 100 mg·kg-1·d-1 DOP (low-dose), 200 mg·kg-1·d-1 DOP (medium-dose) and 400 mg·kg-1·d-1 DOP (high-dose), control group and cisplatin group were intragastrically administrated with equal volume of saline. The mice were sacrificed after DOP treatment for 35 d, and were sampled to evaluate effects of DOP on the number of sperm, sperm quality, histopathology, as well as oxidative stress of testicular tissue in mice. RESULTS Compared with cisplatin group, index of testis and epididymis, the number of sperm, sperm motility and viability were significantly increased (P<0.05 or P<0.01), and sperm abnormality rate was significantly decreased (P<0.01) in cisplatin-LD/MD/HD-DOP group. The SOD activity was significantly increased (P<0.01), and MDA content was significantly decreased (P<0.01) in testicular tissue. Meanwhile, histopathologicla damage of testicular tissue was significantly improved in cisplatin-LD/MD/HD-DOP group. Compared with cisplatin-LD-DOP group mice, the number of sperm was increased and sperm quality was improved, oxidative damage of the testicular tissue and histopathological damage were significantly improved in cisplatin-MD-DOP group and cisplatin-HD-DOP group mice. CONCLUSION DOP could significantly increase sperm numbers as well as improved sperm quality, and promote the antioxidative ability of testicular tissue in cisplatin model mice, the result shows that DOP has preventing effects on reproductive damage of model mice in dose-dependent manner.
关键词
铁皮石斛多糖 /
顺铂 /
生殖损伤 /
精子 /
睾丸
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Key words
Dendrobium officinale polysaccharide /
cisplatin /
reproductive damage /
sperm /
test
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中图分类号：
R965
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参考文献
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脚注
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基金
环保公益性行业科研专项资助(201509044)
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